Services offered by ProteoRed ProteoRed-ISCIII offers a broad range of services for the separation, identification, characterization and quantification of proteins and proteomes. These include the following techniques: Protein Identification Analysis of enzymatic protein digestions by using mass spectrometry (includes: sample preparation, digestion, clean up, and data analysis)
  • Protein Identification by LC-MS/MS (short gradients, <60 min)
  • Protein Identification by LC-MS/MS (medium gradients, <180 min)
  • Protein Identification by LC-MS/MS (long gradientes, >180 min)
  • Protein identification by LC-MALDI (medium gradients, <180 min)
  • Protein Identification by PMF MS - MS/MS by MALDI TOF/TOF
Protein Quantification Relative quantitation of differences in protein abundance by mass spectrometry (includes: sample preparation, labeling, digestion, clean up)
  • Protein Quantification by LC-MSMS (label-free)
  • Protein Quantification LC-MSMS (isobaric methods: TMT and iTRAQ)
  • Protein Quantification LC-MSMS (metabolic labelling: SILAC)
  • Protein Quantification LC-MSMS (isotopic methods: dimethyl, O18)
  • Protein Quantification LC-MSMS (triple quadrupole, MRM peptides)
  • Protein Quantification LC-MSMS - SWATH (Data Independent Analysis, DIA)
  • LC-MSMS (targeted metabolomics, MRM metabolites)
Protein Characterization Analysis of protein modifications such as phosphorylation, methylation, acetylation, ubiquitination. (includes: sample preparation, digestion and clean up)
  • Large scale Protein Characterization (PTMs, isoforms)
  • Small scale Protein Characterization (PTMs, isoforms)
  • Large scale Protein Identification by "de novo" peptide sequencing
  • Small scale Protein Identification by "de novo" peptide sequencing
Structural Proteomics Analysis of protein interactions and protein structure.
  • Ion mobility mass spectrometry
  • Cross-linking mass spectrometry
Molecular Weight Analysis Tools to determine molecular weight of molecules by mass spectrometry (includes: sample preparation and clean up)
  • Molecular weight analysis by MS-MALDI TOF
  • Molecular weight analysis by ESI (Low resolution)
  • Molecular weight analysis by ESI (High resolution)
Electrophoresis Separation of proteins based on their electrical and mass properties
  • 2D Electrophoresis (big gels, No fluorescent)
  • 2D-DIGE
Bioinformatics
  • Image Analysis (2DE, 2D-DIGE)
  • MS data analysis (quantitative proteomics)
  • MS data analysis ("de novo")
  • Functional data analysis
Other Proteomics Analysis
  • Biacore
  • SPE-MALDI-TOF MS
  • Top-down proteomics
  • Peptide Synthesis
  • Protein microarray
  • Human recombinant proteins