Services offered by ProteoRed ProteoRed-ISCIII offers a broad range of services for the separation, identification, characterization and quantification of proteins and proteomes. These include the following techniques: Protein Identification Analysis of enzymatic protein digestions by using mass spectrometry (includes: sample preparation, digestion, clean up, and data analysis)
  • Protein Identification by LC/MS/MS (low to large scale)
  • Protein identification by LC-MALDI (low to large scale)
  • Protein identification by PMF MS - MS/MS by MALDI TOF/TOF
Protein Quantification Relative quantitation of differences in protein abundance by mass spectrometry (includes: sample preparation, labeling, digestion, clean up)
  • Protein Quantification by LC-MSMS (label-free)
  • Protein Quantification LC-MSMS (isobaric methods: TMT and iTRAQ)
  • Protein Quantification LC-MSMS (metabolic labelling: SILAC)
  • Protein Quantification LC-MSMS (isotopic methods: dimethyl, O18)
  • Protein Quantification LC-MSMS (triple quadrupole, MRM peptides)
  • LC-MSMS (triple quadrupole, MRM metabolites)
Protein Characterization Analysis of protein modifications such as phosphorylation, methylation, acetylation, ubiquitination (includes: sample preparation, digestion and clean up)
  • Protein Characterization (PTMs, isoforms; in low or large scale)
  • Protein Identification by "de novo" peptide sequencing (in low or large scale)
Protein Characterization Analysis of protein modifications such as phosphorylation, methylation, acetylation, ubiquitination (includes: sample preparation, digestion and clean up)
  • Protein Characterization (PTMs, isoforms; in low or large scale)
  • Protein Identification by "de novo" peptide sequencing (in low or large scale)
Molecular Weight Analysis Tools to determine molecular weight of molecules by mass spectrometry (includes: sample preparation and clean up)
  • Molecular weight analysis by MS-MALDI TOF
  • Molecular weight analysis by ESI (MS-Q-TOF, MS-Ion Trap)
Electrophoresis
  • 2D Electroforesis2D-DIGE
  • IEF Isoelectrofocusing
  • SDS-PAGE
  • Isoelectrofocusing OFF-GEL
Bioinformatics
  • Image Analysis (2DE, 2D-DIGE)
  • MS data analysis (quantitative proteomics)
  • MS data analysis ("de novo")
  • Functional data analysis
Other Proteomics Analysis
  • Biacore
  • SPE-MALDI-TOF MS
  • Top-down proteomics
Sample Preparation/Clean-up/Other
  • Desalting and concentration (ultrafiltration, reverse phase)Digestion (standard in solution, gel)
  • Digestion (custom: FASP, deglycosylation, alternative enzymes)
  • Fractionation by HPLC (reverse phase, ion exchange)
  • Fractionation by SPE (reverse phase, ion exchange)Fractionation using spin columns (reverse phase, ion exchange)
  • Gel Staining (Coomassie, Fluorescent, Silver)
  • Gel Scanning Fluorescence (Typhoon or similar)
  • Immunoprecipitation of Ubiquitinated peptides
  • Phosphopeptide enrichment (IMAC or TiO2)
  • Protein Depletion (1-2, 12-14, 20, by HPLC)
  • Protein Depletion (1-2, spin column)
  • Protein extraction
  • Protein quantification (Bradford, BCA, etc)
  • Protein precipitation